Carbon-Source-Dependent Toxicity of Carbon Dots: An Environmental Evaluation Using Brine shrimp

Carbon dots (C-Dots) have attracted significant interest due to their strong photoluminescence, aqueous stability, and tunable surface chemistry; however, their environmental safety remains incompletely understood. In this work, C-Dots were synthesized via a rapid microwave-assisted method using two different carbon precursors, D-glucose and ascorbic acid, with ethylenediamine as a passivating agent. The resulting nanoparticles exhibited predominantly amorphous structures with sizes below 10 nm, characteristic absorption bands at ~280–330 nm, and blue photoluminescence centered at ~450 nm. Acute toxicity was evaluated using Brine shrimp at concentrations ranging from 10 to 2000 ppm after 24 and 48 h of exposure. C-Dots synthesized from ascorbic acid showed significant toxicity at 2000 ppm, inducing higher mortality rates after 24 h, whereas D-glucose-derived C-Dots exhibited minimal toxic effects under the same conditions. These findings demonstrate that carbon precursor selection plays a critical role in determining the environmental toxicity of C-Dots and highlight the importance of precursor-dependent design strategies to minimize potential ecological risks associated with carbon-based nanomaterials.