Continuous evolving humanoid for advanced cellular models

Drug discovery research faces significant challenges, with around 90 % of candidate drugs failing in clinical trials. Traditional animal models do not always accurately reflect human physiology and pathology, leading to low predictability of clinical outcomes. In vitro human model using induced pluripotent stem cells (iPSCs), offers a potential solution, but long culture periods and variability among experiments have prompted a shift towards automation in human biomimetic research. We optimized the automated cell culture system "Maholo LabDroid" to establish a platform for the long-term culture of undifferentiated iPSCs and for conducting complex cell-based assays. The Maholo LabDroid, a humanoid robotic system with excellent reproducibility, allows for the addition of peripherals and devices to execute new protocols. To enhance the use of iPSCs, we addressed limitations in detecting real-time changes in cells, supplying sufficient high-quality cells and handling 3D cell models that reflect more physiologically relevant conditions. We integrated a robot arm-assisted imaging system for real-time observation, introduced Bluetooth-enabled electronic pipettes for flexible liquid handling, and expanded the types of culture containers used. These improvements enabled us to determine optimal culture conditions, obtain sufficient quantities of cells, and automatically generate organoids with physiological functions. Using the LabDroid Maholo, we built a platform technology for the provision of advanced cellular models. This platform has the potential to enhance drug discovery research and contribute to the development of cell therapy products.