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The agroindustry, a significant contributor to global economic growth, generates substantial quantities of organic waste due to crop residues, biomass, and processing by-products. This chapter focuses on the potential of agroindustry- generated wastes as valuable sources for the production of bio-based and biosurfactants addressing environmental and economic concerns. Bio-based surfactants, derived from renewable resources, offer an eco-friendly alternative to traditional surfactants, presenting a sustainable solution with diverse applications spanning across agriculture, cosmetics, and industrial processes. The chapter delves into the types and composition of agroindustry wastes, extraction methods, and production processes for biosurfactants. The diverse chemical composition of agro-wastes provides a wide array of precursors for biosurfactant synthesis. Lipids, polysaccharides, and proteins found in these wastes can be selectively extracted and transformed into biosurfactants through various biochemical and chemical processes. Extraction techniques may include enzymatic hydrolysis, microbial fermentation, and chemical treatments, allowing for the efficient isolation of surfactant-active compounds from the complex matrices of agro-wastes. Additionally, the diverse applications of these biosurfactants in different sectors were assessed. By converting these residues into value-added products, the agroindustry not only minimizes environmental impacts associated with waste disposal but also contributes to the development of a more sustainable and eco-friendly industrial landscape. Different agro-wastes contribute distinct characteristics to the biosurfactants they yield. This diversity allows for the customization of biosurfactants meeting specific industrial needs. This chapter contributes to sustainable practices by transforming agroindustry wastes into high-value, green sustainable solutions for various industries and proposing future avenues for research to enhance the efficiency and applicability of bio-based surfactants. Thus, the utilization of agro-wastes for biosurfactant production aligns with the principles of circular economy and sustainable agriculture.