Comprehensive transduceromic profiling of NOP receptor ligands at different Gα subunits

This study provides a comprehensive fingerprint of NOP receptor pharmacology at the single-transducer level. The lack of marked G protein bias at the NOP receptor, more pronounced than for the classical opioid receptors, may reflect a combination of intrinsic receptor properties and assay limitations. These findings underscore the need for innovative chemotypes and complementary experimental strategies to fully resolve potential transducer bias at the NOP receptor.