A conserved bioelectrical signature defines subventricular zone-derived human fetal neural stem cells and tracks their differentiation state

SVZ-derived hfNSCs possess a reproducible bioelectrical signature within the 15-16-week gestational window across independent donors, supporting electrophysiological profiling as a quantitative functional benchmark for identity and standardization. During <i>in vitro</i> differentiation, Vm hyperpolarization and I_A/I_K remodeling track early functional progression, whereas the late emergence of inward currents in only a subset of cells indicates increased heterogeneity at later stages. Overall, these findings support bioelectrical profiling as a quantitative functional biomarker with potential utility for standardization and quality assessment in hfNSC-based regenerative therapies.