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Trichomoniasis is one of the most common protozoal infections of the human urogenital tract and represents a significant global public health problem. The disease is caused by the protozoan parasite Trichomonas vaginalis, which is transmitted primarily through sexual contact and affects both women and men. According to the data of the World Health Organization, millions of new cases of trichomoniasis are registered worldwide every year, indicating the high prevalence and medical importance of this infection. The disease may lead to various complications, including inflammatory disorders of the urogenital system, infertility, pregnancy complications, and increased susceptibility to other sexually transmitted infections. Accurate laboratory diagnosis plays a crucial role in the timely detection, treatment, and prevention of trichomoniasis. Modern microbiological diagnostic methods are based on direct microscopic examination, cultural identification, antigen detection, and molecular biological techniques. Each diagnostic approach has specific advantages and limitations in terms of sensitivity, specificity, cost, and availability in clinical practice. Therefore, a comprehensive laboratory diagnostic strategy is essential for improving the accuracy of detection and reducing the risk of misdiagnosis. The purpose of this study is to analyze modern laboratory diagnostic methods for trichomoniasis, evaluate their effectiveness, and determine their role in clinical microbiology and infectious disease control. The study is based on the analysis of reliable scientific literature and microbiology textbooks that describe the biological characteristics of the pathogen, diagnostic approaches, and their practical application in laboratory medicine. The findings of this research highlight the importance of accurate laboratory identification of the causative agent for effective treatment and prevention of trichomoniasis.