P02.08 Precise and flexible formulation and filling of CAR T cells in a fully closed and automated system - the new CliniMACS Prodigy TCTf process

Conclusion hAB serum is a highly complex component that provides various nutrient sources during CAR T cell manufacturing.While reducing its availability results in comparable culture properties, sc-sequencing identified DEG related to metabolism.However, the SCENITH protocol and mitochondrial mass did not support changes in translational and energy metabolism.Yet, other metabolism pathways remain to be tested to better understand the effect of hAB serum.