Advanced Phosphoproteomics Depth and Quantitative Performance Using an Integrated noFAIMS–FAIMS Approach

This study demonstrates that an integrated noFAIMS-FAIMS approach significantly enhances phosphoproteomic depth and quantification by leveraging the complementary advantages of each method. By capturing unique phosphopeptides from each analysis, this strategy can be a practical and efficient phosphoproteomic approach, providing deeper insights into cellular signaling pathways.