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The issue of the quality of medical education in Ukraine has acquired nationwide significance. In the context of the COVID-19 pandemic, the full-scale war, and the rapid development of artificial intelligence, there is a growing need to adapt teaching approaches in medical universities to contemporary challenges, taking into account the current state of physicians’ professional training. The objective: to analyze perceptions of the current quality of higher medical education by students, interns, and young physicians. Materials and methods. A two-stage survey using an online questionnaire was conducted in 2023 (300 participants, including students and interns) and in 2025 (450 participants, including students, interns, and young physicians with up to 5 years of clinical experience). The respondents represented leading medical higher education institutions (MHEI) in Ukraine and assessed their perception of the current state of higher medical education using a 10-point scale according to the following criteria: human resources capacity, material and technical base, methodological support, integration of innovative technologies, international cooperation, and the level of implementation of modern clinical guidelines and standards (protocols) of medical care in the educational process. Results. Comparison of data from 2023 and 2025 revealed a statistically significant increase in scores related to the integration of innovative technologies into the educational process of MHEI and the development of international cooperation, indicating successful digitalization of education and adaptation of academic mobility programs in recent years. At the same time, a significant decrease in scores was observed for human resources and methodological support, as well as for the level of material and technical infrastructure. The most pronounced gap was identified in the assessment of the implementation of clinical guidelines and standards in the educational process among different respondent groups (from 5.12 ± 0.17 points among students to 2.10 ± 0.12 points among young physicians), highlighting insufficient development of skills related to understanding clinical and legal requirements of real clinical practice at the undergraduate education stage. Conclusions. Based on the survey of students, interns, and young physicians, a positive trend was identified in the development of innovative technologies and international cooperation in MHEI, along with a statistically significant decline in assessments of human, methodological, and material resources. Particular attention is drawn to the low level of practical use of clinical guidelines and standards of medical care reported by interns and young physicians who, unlike students, are engaged in practical medicine. The need to modernize educational programs by expanding elective courses and studying sector-specific regulatory documents from the undergraduate level is substantiated.