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BACKGROUND: This work addresses the problem of determining the time of death of corpses with signs of putrefaction. Specifically, it examines the spectrophotometric analysis of putrefactive blister fluid, followed by the development of a mathematical expression and its use as an additional method. This will improve the accuracy and objectivity of forensic medical reports regarding the duration of the postmortem period. AIM: To determine the possibility of objectifying the diagnosis of the time of death using the spectrophotometric method, for which purpose to study and mathematically describe the dynamics of changes in the optical density of the fluid of the putrefactive bladder on corpses in the late postmortem period. METHODS: Biological material—fluid from a putrefactive bladder—was collected from male and female cadavers of various ages, with evidence of putrefactive biotransformation, at various stages of the postmortem period. Spectrophotometric analysis of the material was performed at the Udmurt Republic Budgetary Healthcare Institution "Bureau of Forensic Medicine of the Ministry of Health of the Udmurt Republic" from February 2023 to July 2024 using an SF-2000 apparatus in the spectral range of 490-520 nm, according to a method developed by the authors. RESULTS: Using optical density data from 77 cadavers, the dynamics of changes in the studied parameter were recorded depending on the time elapsed since death. This allowed us to construct a mathematical expression based on a power-law model with an approximation coefficient greater than 95% for the 24-120-hour postmortem interval. The margin of error for the developed equation was also calculated and a corresponding mathematical inequality was created. The results were validated using a blind experiment on real-world data. CONCLUSION: The application of the obtained results makes it possible to use the quantitative parameter of the putrefactive bladder in diagnosing the time of death. However, given the dynamics of changes in optical density over time, the developed mathematical model can be used in the 24-120 hour postmortem period as an additional method for objectively determining the time of death in the practice of a forensic physician.