Standardized and Pharmacological Evaluation of Herbal Gel for Anti-Inflammatory Activity

Objectives: - The present study was carried out to standardized and pharmacological evaluation of herbal gel for anti-inflammatory activity. Material and Methods: - Herbal extract of plants which contains curcumin, Plumbago zeylanica, Terminalia Billerica and linoleic acid are the active constituents responsible for Anti-inflammatory effect. Extraction of the plant material was done by different solvents. TLC was done to confirm the active constituents present in polyherbal. The in-vivo anti-inflammatory activity of polyherbal extract showing promising COX-2 inhibition was assessed using carrageenan and Phorbol Myristate Acetate (PMA) induced mice oedema animal model. Results: - The percentage yield of extracts were found to 68.9, 75.4, 65.8. The Rf value of polyherbal extract was found to be 0.94. In vivo anti-inflammatory study shows herbal extract had a significant impact on inhibition of oedema formation. The cytotoxicity evaluation study of ethanolic fraction of herbal extract have no effect on cell viability. HPTLC fingerprint of flavonoids of the herbal extract was also prepared as a measure of quality control. Moreover, the herbal extract can be considered as a resource for searching novel antiinflammatory agents possessing COX-2 inhibition. Conclusion: - The herbal extract showed high extraction yield, confirmed flavonoid fingerprinting, significant in vivo anti-inflammatory activity, no cytotoxicity, and promising potential as a safe COX-2 inhibitory antiinflammatory agent.