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The diploma thesis deals with the issue of arboretums and especially their creation. The author focuses mainly on the layout of these gardens in terms of landscape architecture. The problem nowadays is that gardens don‘t initially have some kind of uniform concept, on the basis of which the gardens would be gradually realised. The literary research deals with the importance, history, current situation and possibilities of using arboretums. The work also deals with the possibilities of creating these gardens. For example, how to fulfill as efficiently as possible several of the mission of gardens of a given type. The subsequent analysis of Czech and foreign realizations of these gardens was intended to describe their mission and define the compositional principles of the given premises. On the basis of literary research and analysis of reference realizations, theoretical basis for the creation of arboretums was established. This should serve as a general tool in the process of designing these units. On the basis of previous findings, a proposal for an arboretum has been developed for the study phase. The area addressed was first thoroughly analyzed, then a concept was created and finally a design of the whole premises was drawn up with detailed development of some segments.