Additional file 5: Figure S4. of Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells

HP1β is highly expressed in ESCs. a Western blot for HP1β (top panel) and histone (H3, middle panel) in MEFs and mouse ESCs (R1). For both cell types, the two nuclear fractions that include the whole amount of HP1β and histone H3 (S3 nucleoplasmic fraction, P3 chromatin-bound fraction) are shown. Protein staining with PonceauS (bottom panel) of the blot was used as a loading control. b Total HP1β levels (S3 + P3) were quantified in MEFs versus ESCs from three western blot experiments and normalized to H3 levels; error bars represent standard error of the mean. c Western blot for HP1β in ESCs (R1) and embryoid bodies (EBs) after 7 days of differentiation. Protein staining with PonceauS in the HP1 range of the blot was used as a loading control. d Global view of the expression level of CBX1/HP1β in human pluripotent cells (hESCs and hIPSCs) and in differentiated cells produced by the Amazonia! tool from public human transcriptome datasets [56]. (PDF 255 kb)