BLASTx analysis of assembled insect SRA libraries - part 1

To identify insect species potentially associated with Ophiocordyceps-related yeast-like symbionts, transcriptomic SRA reads were aligned against the StYLS coding sequences of 18 ribosomal proteins and cytochrome c oxidase subunit I (COI). Read mapping was performed with Magic‑BLAST v1.7.0. Libraries with ≥20 aligned reads were downloaded. Reads were then subjected to quality control using the BBTools suite and assembled with SPAdes v4.2.0 in “meta” mode using default parameters. The resulting transcripts were queried against the NCBI core-nr database using DIAMOND BLASTx to identify fungal transcripts belonging to genera within the family Ophiocordycipitaceae. The output files from the BLASTx analysis of the first 100 assembled libraries are provided (please see for 10.5281/zenodo.18506951 the other 43 output files). Sequence similarity searches were performed using a customized tabular output (-outfmt 6) with the NCBI search metrics reported in this order: qseqid qlen sseqid stitle slen pident length mismatch gapopen qstart qend sstart send evalue bitscore qcovhsp scovhsp full_qqual sscinames sphylums.Filenames contain the insect species name and the NCBI accession number of the analyzed SRA library.