Additional file 2: Figure S2. of Heterochromatin Protein 1β (HP1β) has distinct functions and distinct nuclear distribution in pluripotent versus differentiated cells

Mitosis in WT, HP1α KO, and HP1β KO ESCs. a Confocal images of single mitotic nucleus of WT ESCs (top), HP1α KO ESCs (middle), and HP1β KO ESCs (bottom) in metaphase or anaphase immunostained for H3K9me3 (red) and counterstained with DAPI (blue). The merged images shown on the left allow visualization of the DNA and H3K9me3 distribution during metaphase and chromosome segregation in anaphase. Scale bars = 7 μm. b HP1β knockdown experiment (siRNA) in R1 ESCs. Cells were treated with control siRNA or HP1β siRNA and grown for an additional 48 h before cell fixation and immunostaining with HP1β, Nestin, and DAPI. (PDF 2.41 mb)