Improving the efficiency of artificial insemination in high-yielding cows with immunological infertility

Relevance. Currently, in dairy farming, cases of infertility in highly productive cows are being diagnosed with increasing frequency. Accelerated selection requires a comprehensive analysis of reproduction in order to improve its efficiency. Methods. The research was conducted and the results were tested on farms in the Moscow and Oryol regions. The studies involved 52 service bulls and 246 cows, 72 of which had been previously diagnosed with “immune infertility.” Results. A new diagnostic protocol was developed, which improved the effectiveness of artificial insemination in highly productive, genetically valuable cows with a history of more than 6 unsuccessful insemination attempts over 8 or more months. The basis of the protocol is the individual selection of service bulls by titrating their native semen in the alloserum of each problem cow. For individual selection, we used bulls that had undergone a complete andrological examination and had no titers of sperm autoantibodies in their own blood serum. The development of the method included the preparation and execution of artificial insemination, and the determination of pregnancy via ultrasound examination. Conclusions. A solution to the problem of immunological infertility in repeatedly unsuccessfully inseminated cows with no clinical gynecological pathologies is presented. Testing of this protocol resulted in pregnancy in 44.4% of cases in barren cows with a preliminary diagnosis of immune infertility.