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<h3>Background and Importance</h3> When a patient is admitted to hospital, medication changes often occur. Furthermore, the responsibility for the use of the medication switches from patient to nurse. At discharge the patient is less comfortable in using medication possibly resulting in medication errors, reduction of adherence and discomfort for the patient. Self-Administration of Medication (SAM) can help patients in the use of their medication and contributes to a smoother discharge from hospital to home. At this moment it is unclear which patients are eligible to use SAM and this unclarity hinders implementation of SAM in hospitals. <h3>Aim and Objectives</h3> The objective of this study is to assess the proportion of hospitalised patients eligible for SAM and to identify which patient-related and clinical factors may influence the use of SAM. <h3>Material and Methods</h3> A descriptive, cross-sectional flash mob study was conducted in 35 hospitals in different departments in the period of 18-22 November 2024. Eligibility for SAM was assessed by nurses using a 7-question screening tool. The primary outcome was the proportion of patients eligible for SAM. Eligibility was expressed as a percentage using descriptive statistics. Uni- and multivariable logistic regression was used to analyse the association between eligibility for SAM and age, sex, number of medications, use of medication roll, opioid use, educational level, hospital type and ward type. <h3>Results</h3> Of the 2,311 included patients, 1,403 (60.7%) were eligible for SAM. Eligibility rates for SAM varied significantly across patient groups. Women showed higher eligibility (63.5%) (adjusted OR: 1.35 (95% BI: 1.12 – 1.62)) compared to men (58.3%) (reference). The eligibility rate decreased with age: 70.6% (18-67 years) (reference), 62.1% (68-80 years) (adjusted OR: 0.87 (95% BI: 0.70 – 1.09)) and 46% (> 80 years) (adjusted OR: 0.59 (95% BI: 0.46 – 0.77)). The eligibility rate differed between department type: departments with non- elective admissions (61.4%) (reference) versus departments with elective admissions (74.5%) (adjusted OR: 1.75 (95% BI: 1.40 – 2.19)) and high intensity care departments (31.3%) (adjusted OR: 0.37 (95% BI: 0.28 – 0.49)). <h3>Conclusion and Relevance</h3> A large proportion of hospitalised patients are eligible for SAM. These results support wider implementation of SAM in hospitals. <h3>Conflict of Interest</h3> No conflict of interest