A comparative study of invA and ttrC-based real-time PCR assays for the genus-specific molecular identification of Salmonella enterica

Our findings suggest that both the ttrC-based and invA-based PCR assays are equally reliable for the genus-specific identification of a wide Salmonella enterica strain panel of epidemiological relevance and are in complete agreement. However, considering the genomic variability in some Salmonella serovars, a dual-target PCR approach is recommended to potentially improve diagnostic accuracy.