A kingdom-wide full-length RNA atlas reveals the evolutionary landscapes of plant poly(A) tails

Our study provides the first comprehensive poly(A) tail dataset spanning all major lineages of plant evolution, uncovering lineage-specific poly(A) tail length distributions. Notably, we reveal the presence of poly(A)-less transcripts in several plant clades, which is overlooked in previous transcriptomic studies. This atlas offers a valuable resource for understanding the evolutionary and regulatory complexity of mRNA 3' end formation across the plant kingdom.