Search for a command to run...
This record contains a data table summarizing the metatranscriptomic dataset of AE2504 exometabolite incubation experiments. These experiments were conducted on the RV Atlantic Explorer cruise near the Bermuda Atlantic Time Series station in March 2025. The incubation setup consisted of spiking one of two mixtures of 10 compounds representing the most abundant exometabolites excreted by cultures of Prochlorococcus marinus and Micromonas commoda into seawater just collected by CTD cast at 5 m depth. The "exometabolite" spikes contained the compounds in their expected exometabolomic proportions and at expected relevant environmental concentrations. The purpose of the experiment is to understand how natural heterotrophic microbial communities metabolize phytoplankton exudates. To this end, metatranscriptomic samples were collected from seawater samples before incubation and from incubations lasting 6.5 and 12 h. Metatranscriptomes were computationally processed using a bioinformatic workflow described in the attached word document. Briefly, ORFs from co-assembled contigs were taxonomically and functionally annotated. The abundances of ORFs in metatranscriptomic samples were determined by read mapping to contigs. Each row of the attached table contains information on one ORF, including annotations and sample abundances. Please do not publish or present any analysis of these data without permission from Claire Garfield or Mary Ann Moran, who can be reached at claire.garfield@uga.edu and mmoran@uga.edu. Collaborations welcome!