Evaluation of metabolomic changes in serum and intestinal content of morbidly obese patients subjected to bariatric intervention - part 2 Bile acids analysis

<p class="MsoNormal">Obesity, a major health condition in the modern world, poses numerous health risks and can reduce life expectancy by 8 to 10 years. The most effective treatment for obesity remains bariatric surgery, which results in significant weight loss and improved metabolism. Currently, few surgical techniques are performed, and the choice of the procedure is dependent on the age of the patient, the presence and severity of co-morbidities, and the BMI. Some of the most commonly performed bariatric procedures are laparoscopic sleeve gastrectomy (LSG) and one anastomosis gastric bypass (OAGB). Both techniques are effective in inducing weight loss and improving markers of obesity-related diseases, as well as overall quality of life. Although the clinical outcomes and differences between bariatric procedures are well established, data on procedure-specific metabolic alterations remain limited. Many studies indicate an increase in circulating BA after various types of BS. This is due to the changes in anatomical structure, but also as a result of an altered intestinal microbiota. However, this effect is not fully understood in the long term. Therefore, the aim of our study was to validate an analytical method and apply it to the analysis of serum bile acids in female patients after both LSG and OAGB. By including up to a 9–12-month follow-up and a group of healthy, non-obese women as controls, we aimed to obtain a broader understanding of the long-term metabolic changes occurring post-surgery.