WCN26-2673 5-HYDROXY-1-METHYLHYDANTOIN (NZ-419), AN ENDOGENOUS ANTIOXIDANT, ATTENUATES MYCOTOXIN-INDUCED TOXICITY IN HUMAN KIDNEY PROXIMAL TUBULAR CELLS

Ccl19, and Tnfsf13b (encoding BAFF) by fibroblasts within TLSs and reduction of GCB in injured kidneys.Edu assay showed reduced lymphocyte proliferation in residual TLSs.Similarly, anti-CD153 antibodies reduced SA-T cells, diminished TLSs, and ameliorated fibrosis and tubular injury in aged injured kidneys.scRNA-seq of human kidneys with TLSs to investigate counterparts of murine SA-T cells revealed follicular helper T (Tfh)-like cells with high TNFSF8 (encoding CD153) expression.Immunostaining and ISH identified TNFSF8+ T cells within human TLSs, which were considered potential counterparts of murine SA-T cells.Conclusion: These findings demonstrated that SA-T cells within TLSs are essential for TLS maintenance, potentially via driving chemokine/ cytokine production by proinflammatory fibroblasts, and their interactions may sustain lymphocyte proliferation.Renoprotective effects of anti-CD153 antibodies via reduction of SA-T cells and TLS size and the presence of TNFSF8+ Tfh-like cells in human renal TLSs suggested that CD153-directed therapy may be a novel strategy to prevent AKI to CKD progression in the elderly patients.This work was first presented at ASN Kidney Week 2024 and resubmission is permitted by ASN.I have no potential conflict of interest to disclose.I did not use generative AI and AI-assisted technologies in the writing process.