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Durum wheat (Triticum turgidum ssp. durum) is an economically significant cereal crop whose enhancement requires enhanced knowledge on its genetic diversity especially regarding quality-related characteristics. The objective of the current research was to measure genetic diversity in twenty durum wheat accessions in the form of seed storage protein profiling by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Separations were done using 15 percent polyacrylamide gels and genetic variation was measured on the basis of presence, absence, and strength of protein bands. The electrophoretic comparison showed both monomorphic and polymorphic banding patterns and this suggested that there was a high degree of biochemical diversity in the germplasm. Four accessions were monomorphic, and the rest of the accessions had evident polymorphism with strong variation seen in accessions 13-29New-SH7, 13-37New-SH11, 13-61New-SH24 and 13-70New-SH28. A predominant type of polymorphism was observed in low molecular weight glutenins and gliadins in the middle and low range of molecular weights, which are closely related to the strength and quality of the process. Fluctuations in band intensity also indicated the joint action of the genetic background and environmental factors on the accumulation of proteins. In general, the findings reveal that SDS-PAGE is an easy, precise, and inexpensive method of determining genetic diversity of durum wheat. The polymorphic accessions identified can be utilized as great genetic resources in breeding programs so as to improve the quality of the grain, increase the genetic base and preserve the germplasm of durum wheat.