Validation of a Spatially Resolved Reflectance Imaging System for Recovery of µa and µs′ in Absorbing Turbid Media

Many biomedical applications rely on the accurate recovery of absorption and scattering properties of human tissue. These characteristics serve as useful diagnostic indicators, holding information regarding the health and physiological status of a human subject. Many experimental methods exist for the determination of these optical properties, though many, such as integrating sphere methods, are not easily used in an in vivo setting. We have constructed and validated a spatially resolved reflectance imaging system that can be used to measure the absolute optical properties of absorbing turbid media in a non-contact, non-invasive fashion. We present detailed calibration procedures that consider our unique incident beam profile and system response with quantitative comparisons between experimentally and computationally obtained reflectance using Monte Carlo methods. Using highly scattering sphere suspensions with added absorption by ink, we show the spatially resolved reflectance imaging system’s ability to recover absorption within 20% of reference collimated transmission measurements and reduced scatter within 6% of those obtained by an extensively tested integrating sphere system, validating our system in preparation for in vivo measurements of the optical properties of human skin.