rCRUX Generated MiFish Reference Database

rCRUX-generated reference database using NCBI nt blast database downloaded in July 2024. Primer Name: MiFishGene: mitochondrial 12S rRNALength of Target: ~163–185 Primer Reference: Miya, M., Sato, Y., Fukunaga, T., Sado, T., Poulsen, J. Y., Sato, K., Minamoto, T., Yamamoto, S., Yamanaka, H., Araki, H., Kondoh, M., & Iwasaki, W. 2015. MiFish, a set of universal PCR primers for metabarcoding environmental DNA from fishes: detection of more than 230 subtropical marine species. Royal Society open science, 2(7), 150088. https://doi.org/10.1098/rsos.150088 MiFISH-U-F, 5’-3’: GTCGGTAAAACTCGTGCCAGC MiFISH-U-R, 5’-3’: CATAGTGGGGTATCTAATCCCAGTTTG Default rCRUX parameters were chosen for get_seeds_local() and blast_seeds().