Protocol to explore olfactory placode morphogenesis using an agent-based model

Mathematical modeling is a potent tool for determining and assessing reasonable developmental hypotheses. Here, we present a protocol to investigate how variations in chemotactic source distribution influence the formation of bilateral olfactory cell rosettes during zebrafish development. We describe steps for setting up parameters and modifying and testing the different chemotaxis forces. We provide instructions to install and run the code and visualize the results from the GitHub repository. For complete details on the use and execution of this protocol, please refer to Zilliox et al.¹.