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Background: Hospital acquired infection (HAI) is a major health problem today. In spite of progress in medical cares, hospital infections among patients are not uncommon. Although it is difficult to assess the exact incidence of hospital acquired infections in our hospitals, ample evidences exist to indicate the related problems. Most common it is observed that the patient comes to the hospital for treatment of particular ailment but has acquires other infection sometimes leading to septicemia, multi system organ failure and death. With the advancement and increase in knowledge of microbiology, the behavior of different microorganism is well understood and there has been invention of various instruments, equipments and chemicals for making such pathogenic organisms ineffective. While using such aids, it is important that proper protocol is maintained for the effective results. At the same time, one must have proper knowledge of handling and maintaining them in proper condition. ?A study to assess the knowledge of nursing staff towards infection control measures in tertiary Care hospital of selected area?. For the study, the setting of study was ACE Heart and Vascular Institute, Shivalik Hospital Premises, Mohali. ACE Heart and Vascular Institute is Tertiary care health care facility located in Mohali, the heart of Punjab, in North India. Objectives: The study was with an objective to assess knowledge of nursing staff regarding infection control measures and to bring awareness regarding infection control measures. Methodology: A descriptive approach was considered an appropriate for the present study the sample size consists of 50 nursing Staffs non probability convenient sampling technique is used for selection of sample. Results: Study findings showed that 14% staff have good knowledge 62% having average knowledge and 24% having poor knowledge. The finding of the study was concluded that maximum nursing staff belongs to age group of 25 to 30 years, Majority of staff are female, Maximum number of staff had done B.Sc. Nursing, Most of staff are unmarried Maximum number of staff had one to five years of total experience also present unit experience. More number of Staffs was working in the critical area and maximum working in the same area from starting. Conclusion: The infection control knowledge among the nurses is average knowledge; however there is a wide scope of improvement with regular educational programs trainings or courses.