IN Carta imaging dataset for BBB shuttle siRNA-Cy5 signal dynamics across neuron subcellular compartments (EEA1, RAB11, LAMP1)

The dataset contains IN Carta object-level exports (ObjectData, FieldData, and WellData) organized by compartment (EEA1, RAB11, LAMP1) and experimental group (A, G, H, I, J, K). Mean Cy5 signal intensity was quantified from object-level measurements using local background subtraction (BG3) and aggregated to per-well (per-animal) values. Timepoints include naive, 1 hr, 3 hrs, 24 hrs, and 1 week following single dosing, as well as 1 week following repeat dosing. Dosed animals are the animals that received 10mg/kg of the VHH-Fc-siRNA. Experimental groups are as follows: A = Naive G = 1hr Post Dose 1 H = 3hrs Post Dose 1 I = 24hrs Post Dose 1 J = 1wk Post Dose 1 K = 1wk Post Dose 2 These data correspond to Figure 8D in the associated manuscript. This dataset contains raw and processed imaging data. Analysis code is maintained separately on GitHub: https://github.com/lbustos2026/bbb-shuttle-neuron-compartment-image-analysis