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This study investigated the enhancement mechanisms and optimal mix proportion of basalt fiber (BF) in concrete for ship lock galleries. It focused on improving crack resistance, freeze–thaw resistance, impermeability, and abrasion–erosion resistance under complex hydraulic environments. Single-factor tests first determined the reasonable parameter ranges, which were subsequently used in a three-factor, four-level orthogonal experiment to analyze the effects of the water-to-binder ratio, fiber content, and fiber length on concrete’s mechanical properties. Range analysis of the orthogonal experiment indicated that the water-to-binder ratio was the most dominant factor (R = 57.4), followed by fiber content. Based on this, further durability tests were conducted, including ring restraint cracking, impermeability, freeze–thaw resistance, and abrasion–erosion resistance. Multi-objective optimization was performed using full factorial experiments and a comprehensive performance evaluation system. The final optimal mix proportion was determined as: a water-to-binder ratio of 0.35, a fiber content of 0.2%, and a fiber length of 12 mm. With this mix, the concrete’s ring cracking time was extended by 69.9%, the relative dynamic elastic modulus retention reached 73.0% after 100 freeze–thaw cycles, the relative permeability coefficient was 1.04 × 10−6 cm/h, and the abrasion–erosion resistance strength increased to 7.05 h·m2/kg, which achieved an optimal synergy among the mechanical properties, key durability indicators, and their workability. Mechanism analysis revealed that BF formed a three-dimensional, randomly distributed fiber network that comprehensively enhanced concrete performance through multi-scale mechanisms, including bridging, pore refinement, and energy dissipation. This research has provided systematic experimental evidence and mix proportion support for the durability design and engineering application of BF concrete in ship lock galleries.