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Wound healing is a fundamental process that allows tissues to restore their structure and function after injury. While the broad stages of inflammation, proliferation, and remodeling have been well described, much of our current understanding has come from fixed cells and tissues that miss the dynamic nature of repair. In this chapter, we focus on how live imaging has changed that perspective. By following cells and tissues in real time, live imaging has revealed the dynamics of keratinocyte migration, fibroblast remodeling of the extracellular matrix, and the timely arrival of immune and vascular cells at the wound site. Advances in microscopy, from confocal and multiphoton imaging to intravital approaches, have made it possible to visualize wound healing with spatiotemporal precision in both in vitro and in vivo systems. These techniques have uncovered mechanisms such as coordinated leader–follower migration, lamellipodial crawling, and purse-string closure that cannot be captured by static methods alone. By highlighting these insights, this chapter illustrates how live imaging is reshaping our view of wound healing and opening new avenues for improving tissue repair and regenerative medicine.