Integrated microRNA‑mRNA network analysis of invasive and immune‑inflammatory pathways in sebaceous carcinoma of the eyelid with pagetoid spread

Pagetoid spread is a poor prognostic factor in sebaceous carcinoma (SGC) of the eyelid; however, its underlying molecular mechanisms remain unclear.The present study aimed to investigate the expression profiles of microRNAs (miRNAs/miRs) and mRNAs in SGC of the eyelid with pagetoid spread, compared with cases without pagetoid spread, and to explore potential miRNA-mRNA regulatory networks.Total RNA was extracted from three patients with SGC of the eyelid with pagetoid spread (pagetoid group), three patients without pagetoid spread (non-pagetoid group), and three patients with normal tarsal plate tissue obtained during free tarsal plate reconstruction (control group).Small RNA sequencing was performed using a next-generation sequencer to identify differentially expressed miRNAs among the groups, and bioinformatics analyses of mRNA expression profiles were conducted to determine associated biological functions and construct miRNA-mRNA interaction networks.In the pagetoid group, upregulated mRNAs and downregulated miRNAs were associated with enhanced invasion, migration and immune-inflammatory responses.Three miRNAs, miR-1275, miR-1976 and miR-330-5p, were commonly involved in these functional networks.Conversely, downregulated mRNAs and upregulated miRNAs were associated with impaired lipid metabolism, including miR- 760-3p, miR-1266-5p, miR-3918, miR-1269a and miR-198.These findings suggest that SGC of the eyelid with pagetoid spread demonstrates increased invasive and migratory capacity, reduced lipid metabolic function and enhanced immune-inflammatory responses, which may contribute to its aggressive biological behavior.