From <scp>DNA</scp> Methylation Microarray to Digital <scp>PCR</scp> : A Stepwise Strategy for Tissue Specific <scp>cfDNA</scp> Biomarker Development

This proof-of-concept study outlines a scalable method for developing PCR-based tissue-specific cfDNA biomarkers. Our kidney-specific assay demonstrates the potential for rapid, cost-effective organ injury monitoring and could be adapted for other tissue types. This strategy may support future diagnostic applications in nephrology, oncology, and transplant medicine.