Properties of Nucleotide Pool and Its Relationship with Differentially Expressed Transcriptome and Fibrosis Progression in CCl4-induced Mice

Abstract JOURNAL/jpin/04.03/02275668-990000000-00068/figure1/v/2026-04-02T113239Z/r/image-tiff The properties and dynamics of small biomolecule pools of a cell or an organ have a significant effect on the metabolism of the related biomacromolecules and the ongoing life process. Here, we investigated the properties of the nucleotide pool and differentially expressed transcriptome in the liver of CCl 4 -induced (CI) mice for fibrosis and explored the relationship between the two. We used high-performance liquid chromatography to detect the contents of nucleotide pool in control and treatment groups, while the changed gene expression data in fibrosis progression were collected from BioGPS, and the change ratios of differentially expressed genes were mined out from different literatures about CI mice for fibrosis. All data, expressed as mean ± standard deviation, were analyzed with the SPSS. The main results were as follows: (1) the performance of nucleotide pool of CI livers was quite different from that of control livers, i.e., the contents of the total nucleotides and nucleoside mono phosphates (NMPs, the dominant components of the pool), and the property of nucleotide pool was associated with the physiological state as well as its correlation coefficient was 0.904 ( P < 0.05) and (2) the property of nucleotide pool was associated with the differential transcriptome during fibrosis, the variable quantity of four kinds of nucleotides (NMP) within the nucleotide pool and the counterparts of differentially transcriptome of CI mice have the significantly positive correlation with the correlation coefficient as 0.927 ( P < 0.05). The important connection of the nucleotide pool with the differentially transcriptome and the liver fibrosis was revealed for the first time. The new mechanism of exogenous nucleotides that had an effect on fibrosis was revealed.