SELECTIVE TRANSCRIPTOMIC VULNERABILITY OF MEMBRANE-INTEGRATED ARCHITECTURES DURING NEURAL TISSUE VITRIFICATION

ABSTRACT Cryopreservation is essential for long-term storage of biological tissues. Yet, surprisingly, the precise molecular impact of cryopreservation on tissue transcriptomes remains poorly defined. This study provides the first resource of whole-genome transcriptomic changes following cryopreservation. This study used bulk RNA sequencing to examine how preservation method (snap freezing or vitrification) affects transcriptomes in mouse cerebral cortex and hippocampus. This allowed us to separate cryoprotectant-specific changes from cold induced-changes via snap freezing. In a subset of genes, tissues processed under vitrification conditions showed selective under-representation of a small but structurally coherent group of transcripts, with the hippocampus exhibiting greater vulnerability than the cortex. UniProt annotation revealed that affected transcripts were strongly enriched for proteins with membrane-associated, secretory-pathway, and multi-pass topologies, indicating that structurally complex membrane-integrated architectures are disproportionately sensitive to vitrification. Pathway-level analysis using iPANDA further showed that negative preservation scores in vitrified tissue clustered primarily within signal transduction and metabolic pathways, suggesting coordinated pathway-level disruption rather than global transcript loss. Together, these results demonstrate that vitrification conditions induce selective and structured molecular perturbations in neural tissue, defined by the under-recovery of transcripts associated with membrane and secretory pathway organization. This work highlights molecular vulnerability during vitrification and emphasizes the need for transcript-level evaluation when optimizing cryopreservation approaches for neural systems.