A single-cell multi-omics atlas of human eyelid skin

The skin acts as the first barrier protecting the human body against the external environment. Transcriptional heterogeneity in human skin is widely confirmed, but the regulatory mechanisms remain largely unexplored. In this study, we employed high-throughput single-cell chromatin accessibility and transcriptome sequencing (HT-scCAT-seq), a technique for simultaneous analysis of the transcriptome and epigenome. We used HT-scCAT-seq to analyze 10,065 cell profiles from four adult human eyelid skin and define 13 distinct cell types. In addition, we described detailed molecular signatures and identified key gene regulatory network enriched in each cell type. Our dataset is a valuable resource for further research in human skin biology.