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<b>Purpose</b> Health care workers have been shown to experience a decline in mental health and psychological well-being due to work-related factors. The purpose of this study was to explore the relationship between demographic and work variables, psychological resilience, emotional labor, and employee well-being among clinical dental hygienists.<b>Methods</b> A cross-sectional survey research design was used with a non-probability sample of clinical dental hygienists (n=411) using validated instruments including: the Emotional Labour Scale, Resilience Evaluation Scale and Employee Well-Being Scale. Participants were recruited on dental hygiene social media sites. Descriptive and inferential statistics were used to analyze the results.<b>Results</b> The completion rate was 78% (n=322). The regression model for life well-being (LWB) was statistically significant (adjusted R2=.287, <i>p</i><.001). The predictor that increased LWB was self-confidence (β=.403) while predictors of decreased LWB included surface acting (β=-.246), and hours worked (β=-.179, <i>p</i><.001). The regression model for workplace well-being (WWB) was statistically significant (adjusted R2=.262, <i>p</i><.001). Positive predictors of increased WWB included self-confidence (β=.353, <i>p</i><.001), deep acting (β=.130, <i>p</i>=.007), age (β=.105, <i>p</i><.034) while surface acting (β=-.252, <i>p</i><.001) predicted lower WWB. The regression model for psychological well-being (PWB) was statistically significant (adjusted R2=.398, <i>p</i><.001). Predictors that increased PWB included self-confidence (β=.352, <i>p</i><.001), self-efficacy (β=.217, <i>p</i>< .001), deep acting (β=.162, <i>p</i><.001), and frequency (β=.098, <i>p</i><.035), while surface acting (β=-.193, <i>p</i><.001) predicted lower PWB.<b>Conclusion</b> Dental hygienists displayed high levels of psychological resilience. However, hours worked and the factors impacting emotional labor, such as deep acting and surface acting, negatively impacted employee well-being. More research is needed to investigate strategies for managing emotional labor among dental hygienists.