Vector Field Techniques for Detection of Neuronal Dynamics and Axonal Guidance / Axonal Pathfinding in the Presence of Mercury

The role of mercury in the development of various neuropathologies is well known.Biogeochemical cycles of mercury indicate the possibility of its introduction into various organisms (including humans) through the corresponding trophic chains.However, the literature analysis shows that until recent years there have been almost no works on the dynamic microscopic study (either in situ or in vivo) of the connectome involution in aquatic organisms (including fishes, marine mammals, etc.) under the mercury exposure.In this regard, we attempted to study the dynamics of morphogenesis and breakdown of the emerging connectome upon the mercury exposure.Dynamics of the axonal pathfinding / axonal guidance was carried out in a series of frames with an timecode (address-time code), on which it is possible to track the motion estimation vector fields of displacements and reaction-diffusion processes in the neural cell culture immediately after the introduction of a drop of either mercury or a mercury-containing liquid using capillary micropipettes.This paper describes the effects observed and discusses the possible mechanisms underlying them.