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Among lower-body techniques in karate, the Mawashi Geri Jodan is regarded as the most frequently applied, technically sophisticated, and potentially hazardous skill. Yet, whether karate athletes of varying proficiency levels exhibit differential mastery of this technique remains empirically unexamined. This study aimed to reveal movement control strategies of elite athletes by comparing kinematic and surface electromyography (sEMG) characteristics of Mawashi Geri Jodan between elite and sub-elite female karate practitioners. A total of eight female karate athletes (4 elite, 4 sub-elite) were recruited. During the execution of the dominant-leg Mawashi Geri Jodan, they struck a karate punching bag positioned at head height, while kinematic and sEMG data were synchronously collected. Analyzed metrics included phase durations, center of mass (COM) displacement, joint angles/angular velocities, and integral electromyography (IEMG) with muscle work percentage of 8 lower limb muscles. Independent-sample t-tests were used for intergroup comparisons (α = 0.05). Compared with the sub-elite group, elite athletes completed the full Mawashi Geri Jodan in significantly less time (0.825 ± 0.07 s vs. 1.030 ± 0.05 s, p < 0.01) and exhibited a shorter core striking phase (p < 0.05). Kinematically, elite athletes showed smaller vertical COM displacement during the striking phase (p < 0.05) and greater hip joint range of motion (p < 0.05). sEMG data revealed significantly higher activation of lower limb prime movers (vastus lateralis, gastrocnemius) during the striking phase and greater rectus femoris contribution during the recovery phase in elite athletes. Elite female karate practitioners demonstrate superior movement efficiency, body stability, and neuromuscular coordination in Mawashi Geri Jodan. Technical training should prioritize hip joint flexibility and stability, synergistic explosive force generation of the lower limb kinetic chain during the striking phase, and active rectus femoris activation during the recovery phase to enhance execution precision and efficiency.