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This study investigated the expression profile of the Toll-like receptor 2 (<i>TLR2</i>) gene in goats across three reproductive groups - twin-bearing (<i>n</i> = 12), single-bearing (<i>n</i> = 10) and non-pregnant (<i>n</i> = 8) - using quantitative real-time PCR (qRT-PCR). Descriptive statistics revealed that twin-bearing goats were significantly heavier at service and kidding, with higher litter weights compared to other groups (<i>p</i> < 0.05). No breed-wise differences were observed, allowing pooled analysis for gene expression. Melt curve and amplification plot analyses confirmed primer specificity and efficient amplification. <i>TLR2</i> expression was significantly upregulated in twin-bearing goats (ΔCt = -2.14 ± 0.89), with a mean 4.5-fold increase (<i>p</i> < 0.001) compared to non-pregnant controls, suggesting enhanced immune activation. Single-bearing goats showed negligible and non-significant changes in expression (ΔCt = -0.01 ± 1.45; <i>p</i> = 0.984). Statistical comparisons showed a significant difference in <i>TLR2</i> expression between twin- and single-bearing groups (mean difference: 3.39; <i>p</i> < 0.001). These results reveals a significant association of twin pregnancies with elevated expression level of <i>TLR2</i> gene in resource population. While the functional implications require further investigation, this upregulation may be associated with immune adaptations to increased gestational load. Overall, this study identifies <i>TLR2</i> as a candidate gene expression marker strongly correlated with twin pregnancy in goats. Further validation through longitudinal studies on larger cohorts and investigation of tissue-specific expression is necessary to assess its potential predictive value for reproductive performance in small ruminants.