Comment on egusphere-2026-555

<strong class="journal-contentHeaderColor">Abstract.</strong> We explore geographic scaling of metabolism estimates derived from dissolved oxygen measurements with data from 75 sites in three corresponding ecoregions across the temperate steppes of Mongolia and the United States. We used a nested analysis with descending spatial scales (country, ecoregion, river basin, upper or lower watershed, and wide or constrained valleys) to assess spatial heterogeneity. We then linked estimates of metabolism with reach-to-watershed-scale metrics representing geomorphology, topography, climate, and anthropogenic activity to provide possible explanations for spatial scaling dependencies. We evaluated gross primary production (GPP) and ecosystem respiration (ER) at in-situ water temperature and after standardizing them to 20 &deg;C (GPP₂₀ and ER₂₀). There was no significant effect of scaling on ER, and river basin explained only modest variation in GPP. In contrast, GPP₂₀ varied significantly with ecoregion, river basin, basin position (upper vs. lower), and valley morphology (constrained vs. wide). ER₂₀had no significant spatial predictors. Best regression models for GPP included positive relationships with water velocity and median basin slope and for ER included mean basin air temperature, percentage of urban land use in the basin, and GPP. Best subset regression models for GPP₂₀ included depth, water velocity, and basin slope and for ER₂₀ included depth and mean basin air temperature. The proportion of the watershed in urban or cropland was explanatory of ER, but not GPP. We conclude that fundamental components of ecosystem metabolism respond to different watershed scales and to distinct environmental controls. Thus, macrosystem-scale studies require multi-scale assessment to predict and capture variation in aquatic metabolism. This suggests that universal models of river metabolism are unlikely to perform as well as models built to match the specific scale of inquiry or management.