Cyclodextrin Metal–Organic Framework With Amino Acid Chiral Ligands for Capillary Electrochromatographic Enantioseparation

ABSTRACT Metal–organic frameworks (MOFs) are widely used in the field of chromatographic separation due to their unique properties. A vapor diffusion method was used to synthesize the cyclodextrin MOF (CD‐MOF) with D‐histidine (D‐His) as a chiral ligand (D‐His‐ γ ‐CD‐MOF), which was applied to capillary electrochromatography (CEC). D‐His‐ γ ‐CD‐MOF was characterized by X‐ray diffractometer (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), Fourier transform infrared spectrometer (FT‐IR), and thermogravimetric analyzer (TGA). This CEC system achieved baseline separation of three chiral drugs under optimal separation conditions. In addition, this CEC system was evaluated for repeatability using salbutamol as a model drug and the results showed that this system had good repeatability. Additionally, the chiral separation mechanism of the CEC system was discussed through adsorption kinetic experiments, adsorption isotherm experiments, and adsorption thermodynamic experiments.