From two putative inquilines to one definitive parasitoid: clarifying feeding ecology and species limits in Nearctic Euceroptres Ashmead wasps (Hymenoptera: Figitidae)

Oak galls are structures of modified plant tissue, induced by an ovipositing female oak gall wasp (Hymenoptera: Cynipidae: Cynipini), that provide food and shelter for her progeny. Myriad other wasp species exploit galls, feeding on either the gall wasp larva (parasitoidism), the gall tissue (inquilinism / kleptoparasitism), or other parasites within the gall (hyperparasitism). Though many oak gall-associated wasps are now described, their feeding ecology often remains poorly documented, particularly in the species-rich communities across North America. Wasps in the genus Euceroptres(Hymenoptera: Figitidae) exemplify this problem, long having been assumed to be inquilines based on limited evidence. We used dissections, micro-CT scanning, and DNA barcoding to discover that Euceroptres do not induce hypergalls or modify the internal composition of their host gall. We also found a Euceroptres larvae feeding on gall-inducer larva within an unaltered gall wasp larval chamber. Thus, Euceroptres are parasitoids, not inquilines. While sequencing larval and adult Euceroptres, we also found that the two previously described species in eastern North America, E. whartoni and E. primus, did not separate into distinct clades, suggesting they are a single species. Subsequent morphological comparisons support this finding, allowing establishment of E. whartoni Buffington & Liljeblad, 2008 as a syn. nov. of E. primus Ashmead, 1896.