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Autonomous navigation is essential for orchard transportation robots to support automated operations and precision orchard management. However, in trellis orchards, dense vegetation and complex canopy structures often degrade the stability of GNSS-based navigation in in-row environments. To address this issue, this study proposes a GNSS–vision integrated navigation framework for orchard transportation robots. The performance of GNSS-based navigation in out-of-row environments and vision-based navigation in in-row environments was experimentally evaluated under representative orchard operating conditions. In out-of-row areas, the robot employs GNSS-based path planning and trajectory tracking to achieve reliable navigation in relatively open, lightly occluded environments. During in-row navigation, a deep learning-based real-time object detection approach is used to detect tree trunks and trellis supporting structures. By integrating corner-point selection with temporal RANSAC-based line fitting, a stable orchard row structure is constructed to generate robust navigation references. The visual perception module serves as the front-end sensing component of the navigation system and is designed to be independent of specific object detection architectures, allowing flexible integration with different real-time detection models. Field experiments were conducted under various orchard layouts and growth stages. The average lateral deviation of GNSS-based navigation in out-of-row scenarios ranged from 0.093 to 0.221 m, while the average heading deviation of in-row visual navigation was approximately 5.23° at a robot speed of 0.6 m/s. These results indicate that the proposed perception and navigation methods can maintain stable navigation performance within their respective applicable scenarios in trellis orchard environments. The experimental findings provide a practical and engineering-oriented basis for future research on automatic navigation mode switching and system-level integration of orchard transportation robots.