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This dataset contains raw 12 bit imaging data for a specific field of view (FOV) defined by the coordinates below. The data is organized into "RoughScan" for initial FOV assessment and "focus_stack" for Z-position optimization. # FOV Specifications - Flow Cell: A - Lower Left (x, y): 17.462, 35.5 - Upper Right (x, y): 15.768, 34.252 # Directory Structure The repository contains the following tar.gz archives of - RoughScan.tar.gz: Contains out-of-focus tile images. - focus_stack.tar.gz/: Contains Z-stacks for focus optimization. # Data Descriptions 1. RoughScan.tar.gz This tarball contains initial scans used to visualize the FOV. These images are intentionally out of focus. - Channels: 4 unique channels (558, 610, 687, 740). - Tiles: 3 x tiles (10292, 10607, 10922) per channel. - Metadata: .txt files include specific scan parameters (e.g., meta_RoughScan...). 2. focus_stack.tar.gz (focus_stack/ & focus_stack.tar.gz) This tarball contains a series of images taken at different Z-heights to determine the optimal focus position. - Channels: 4 unique channels (558, 610, 687, 740). - Format: Images are labeled cXXX_fY.tiff, where XXX is the channel and Y is the frame index. - Mechanism: The frame index corresponds to the relative Z-position of the objective as it moves through the focal plane. # Usage Notes - To extract the focus stack: tar -xvzf focus_stack.tar.gz - To extract the rough scans: tar -xvzf RoughScan.tar.gz