STUDY OF A CARDIAC ISCHEMIA MODEL INDUCED BY OXYGEN DEPRIVATION IN PHYSIOLOGICAL SOLUTION

This study aimed to assess the effect of the oxygen deprivation variant in inducing I/R injury in isolated rat hearts using histological and biochemical analyses. The hearts of the control group were perfused with oxygenated KH solution for 120 min, whereas the I/R group developed ischemia with KH solution without oxygen for 30 min. Our histological data provided evidence that the protocol used induced ischemia (Control= 6.20±1.43 vs I/R= 51.07±5.21% of the infarct area). Superoxide dismutase activity did not show significant differences between groups, while catalase exhibited a lower activity in the I/R group (37.56±4.67 U/mg protein) compared to the control group (55.37±4.13 U/mg protein). Lipid peroxidation was doubled in the I/R group (I/R= 4.21±0.49 nmol/mg; Control= 2.24±0.33 nmol/mg protein). A non-significant reduction of the mitochondrial complex I activity was shown in ischemic heart (Control= 1822±352.4 vs I/R= 1584±313.4 nmol.min-1. mg-1protein) and significant reduction in complex II (Control= 2326±156.6 vs I/R= 1512±245.8 nmol.min-1. mg-1 protein).We suggest that the methodology described here is an excellent protocol, in which severe ischemia is associated with stress and mitochondrial damage.