Side-by-Side Systematic Characterization of Novel FPs in Budding Yeast

Fluorescent proteins (FPs) have become indispensable to biological research. Since the discovery of the first FP, <i>Aequorea victoria</i> green fluorescent protein (avGFP), new fluorescent proteins have been continuously being developed. To achieve optimal experimental results, selecting an FP based on specific characteristics─such as brightness, photostability, photochromicity, monomericity, pH robustness, and fluorescence lifetime─is essential. However, a thorough side-by-side comparison of these characteristics is missing for the latest generation of FPs. Here, we provide a comprehensive characterization in yeast of the most recently developed FPs, including FPs that were codon-optimized for yeast. We believe that this provides an excellent compendium for choosing the most suitable FP for research purposes.