Scores Raw Data_Manuscript MHC equine antibodies_A Cequier

This dataset contains the raw data obtained from a microcytotoxicity assay performed using equine mesenchymal stem cells (MSCs) as target cells to detect cytotoxic alloantibodies directed against the major histocompatibility complex (MHC) of donor MSCs. MSCs derived from each donor were tested against serum samples collected from recipient horses at eight predefined time points following two administrations of MSCs. Serum samples were evaluated under three conditions: undiluted (neat), and diluted at 1:2 and 1:16. Cytotoxicity was assessed using a semi-quantitative scoring system based on the percentage of dead cells: grade 1 (<10%), grade 2 (10–19%), grade 4 (20–49%), grade 6 (50–80%), and grade 8 (81–100%). Cytotoxicity scores ≥6 in neat serum were considered indicative of a positive alloantibody response, while scores ≤2 were considered negative. The dataset includes samples from experiments in which MSCs were administered under different biological conditions (chondrogenically differentiated, pro-inflammatory primed, or naïve) and across different donor–recipient MHC compatibility settings. For each condition, the mean cytotoxicity score of technical replicates is reported.