Non-Diabetic Insulin Use in the Treatment of Neoplasms: A Pilot Study on the Insulin Potentiation Technique and p53 Expression

Background: The Warburg effect describes the metabolic shift toward glycolysis in neoplastic cells. Insulin Potentiation Therapy (IPT) leverages this metabolic vulnerability by using insulin to induce a state of therapeutic hypoglycemia. While IPT has been used clinically for decades, its impact on genetic markers remains under-studied. Objective: To evaluate the expression of the tumor suppressor protein p53 and the oncogene c-myc in cancer patients undergoing IPT-induced hypoglycemia. Methods: A pilot study of 10 oncology patients was conducted in a controlled hospital setting. Following informed consent and under hemodynamic monitoring, patients received insulin-induced hypoglycemia. Blood samples were analyzed for p53 and c-myc expression levels via standardized hematological protocols. Results: Preliminary results indicate that therapeutic hypoglycemia serves as a cellular stressor that correlates with an increase in p53 expression and a concomitant decrease in c-myc expression across various tumor types. Conclusion: These findings suggest an epigenetic basis for IPT, where metabolic manipulation influences the expression of key regulators of the cell cycle.