Bite‐ <scp>DNA</scp> Shows Substantial Browsing on Willows ( <i>Salix</i> spp.) by North American Bison in Yellowstone National Park

ABSTRACT Riparian willows ( Salix spp.) in Yellowstone National Park have long been shaped by ungulate browsing, yet the specific contribution of individual herbivore species remains unclear. We applied a bite‐DNA metabarcoding approach, extracting saliva DNA from browsed willow twigs, to directly identify the browsing community across six northern range riparian sites. Mammalian DNA was successfully assigned for more than half of the collected bite samples, revealing browsing by moose ( Alces alces ), North American bison ( Bison bison ), elk ( Cervus canadensis ), deer ( Odocoileus sp.), bighorn sheep ( Ovis canadensis ), and jackrabbit ( Lepus townsendii ). Contrary to the traditional view of bison as primarily grazers, bite‐DNA showed that bison were the most frequent browsers of willows, present at all sites and contributing the majority of browsing bites. Elk, historically considered the primary browser on riparian shrubs, were detected less often, whereas mule deer browsing was consistently recorded and frequently exceeded elk. Browsing height largely overlapped among species and was significantly higher for bighorn sheep than for bison and mule deer. Diameter of browsed twigs did not differ significantly between species. Browsing composition varied locally without clear spatial patterns, suggesting that site‐level factors shape where different ungulates browse willows. Our results demonstrate substantial bison browsing on riparian willows and highlight shifting herbivore impacts on Yellowstone's riparian ecosystems.