Search for a command to run...
The aim of this study was to investigate how delayed fertilization with individual macronutrients (N, P, K, Ca, Mg, and S) affects the growth, yield components, biomass, and spectrophotometric indicators of spring wheat grown under controlled hydroponic conditions. Nutrient deprivation was initiated at BBCH stage 23 and maintained for 21, 28, 35, or 133 days, corresponding to BBCH stages 30, 32, 37, and 99, respectively. In selected treatments, the complete nutrient solution was subsequently restored until harvest to evaluate recovery potential. N, P, and Ca deprivation exerted the strongest negative effects on biomass accumulation across all deprivation durations. Compared to the fully supplied control, biomass was reduced by 60% under N deprivation and by 44.5% under P deprivation after 21 days. After 35 days, calcium deprivation resulted in a 97.7% reduction in biomass. Following 133 days of deprivation, biomass was reduced by 98%, 96.8%, and 95.9% under N, calcium, and P deficiencies, respectively. Root mass followed a similar pattern: after 21 days, it decreased by 52.46% (N) and 36.44% (P); after 28 days—by 57.4% (N) and 52.7% (P); after 35 days—by 90.7% (Ca), 66% (N), and 59.1% (P); and after 133 days—by 95.1–90.1% (Ca, N, P). Magnesium deprivation caused substantial reductions in growth parameters, reflecting its central role in chlorophyll structure and photosynthetic efficiency. Sulfur deprivation resulted in moderate but consistent biomass suppression and spectral divergence, indicating its importance in protein synthesis and redox regulation. Short-term deficiencies allowed partial recovery of growth and productivity, whereas long-term deprivation induced pronounced morphological alterations linked to stress adaptation. These effects were further confirmed through in vivo spectral reflectance measurements compared to healthy control plants.